Preparation, Characterization and In Vitro Cytotoxic Activity of Curcuminoid Extract from Wonogiri's Curcuma Xanthorriza RoxB. Loaded Solid Lipid Nanoparticle in Human Breast Cancer,
ABSTRACT
Wonogiri's Curcuma xanthorriza RoxB., is the medicinal plant that grows in Wonogiri region, contains the highest level of curcuminoid among other regions, and widely used as traditional herbal medicines (Jamu) in Indonesia. Wonogiri's curcuminoid is well known to have wide pharmacological properties particularly in wide range of cancer diseases. However, water solubility and oral bioavailability find very low. Lipid based carrier called by solid lipid nanoparticles (SLN) overcome those of limitations. The purpose of this study is to encapsulate Wonogiri's curcuminoid extract into solid lipid nanoparticles (SLN-Cur-Won) to provide prolonged with improve stability bioavailability, involving non-toxic carrier and to observe their cytotoxic activity in vitro. SLN-Cur-Won was prepared by combining both high temperature high-sheer homogenization and ultra-sonication techniques. The physiochemical characters were obtained by spectrophotometry UV-Vis, particle size analysis (PS), zeta potential analysis (ZP), polydispersity index analysis (PdI) and entrapment efficiency (EE). In vitro cytotoxic activity through MCF-7 cell lines was determined by using MTT assay. SLN-Cur-Won was found to have maximum entrapment efficiency up to 64.67 ± 1.843% and stable at ambient temperature (25 0 C) for 1 month. The cytotoxic activity of SLN-Cur-Won in MCF-7 cells was four time more potent than the parent Wonogiri's curcuminoid extract (Cur-Won). Moreover, the SLN exhibited no toxic effects during observations. Wonogiri's curcuminoid extract loaded solid lipid nanoparticles (SLN-Cur-Won) offer promising as potential chemo-preventive agent in breast cancer therapy.
Keywords: MTT assay, polydispersity index, solid lipid nanoparticle, Wonogiri’s Curcuma xanthorriza RoxB., zeta potential.
Figure 4. Growth Inhibition Activities of Cur-Std, Cur-Won, SLN-Cur-Std, SLN-Cur-Won and SLN on MCF-7 cells after 24 h Incubation (n=3). Results are Presented as Mean ± standard deviation (S.D.)
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